diff --git a/README.md b/README.md index 81a4b80af5f90a525212f294b2eb855e6be3a186..5b106e1f781e1988516ad1adad9ca4a199793ee0 100644 --- a/README.md +++ b/README.md @@ -9,22 +9,22 @@ Original publication: <https://doi.org/10.1371/journal.pone.0229041> Original code: <https://github.com/Hyojung-Choo/Myosoft/tree/Myosoft-hub> -## `1_identify_fibers.py` +## [`1_identify_fibers.py`](1_identify_fibers.py) -- Will identify all fibers based on the membrane staining using WEKA pixel - classification, filter them according to the morphometric gates and save the +- Will identify all fibers based on the membrane staining using [Cellpose](https://github.com/MouseLand/cellpose) segmentation, filter them according to the morphometric gates and save the corresponding ROIs. -- Will now also save the WEKA segmentation as a binary so it can be edited + - Need to be installed ont the machine where the script is run. Follow [this guide](https://wiki.biozentrum.unibas.ch/display/IMCF/Cellpose+python+environment) to create the environment. +- Will now also save the Cellpose segmentation as a binary so it can be edited manually. If you do so, you need to run the "extended particle analyzer" manually as well to choose & apply the morphometric gates. - Can be run in batch. -## `2a_identify_MHC_positive_fibers.py` +## [`2a_identify_MHC_positive_fibers.py`](2a_identify_MHC_positive_fibers.py) - Allows to manual re-run the MHC positive fiber detection. Useful in case you would like to re-run detection with a manual threshold for an image. -## `2b_central_nuclei_counter.py` +## [`2b_central_nuclei_counter.py`](2b_central_nuclei_counter.py) - Will identify centralized nuclei given a ROI-zip together with its corresponding image. @@ -32,14 +32,14 @@ Original code: <https://github.com/Hyojung-Choo/Myosoft/tree/Myosoft-hub> information of a MHC staining channel. - The ROI color code is annotated in the results table. -## `2c_fibertyping.py` +## [`2c_fibertyping.py`](2c_fibertyping.py) - Identifies positive fibers in up to 3 channels given a ROI-zip together with its corresponding image. - Includes identification of double and triple positive combinations. - The ROI color code is annotated in the results table. -## `3_manual_rerun.py` +## [`3_manual_rerun.py`](3_manual_rerun.py) - Requires an already open image with an already populated ROI manager. - Allows to manually select measurement parameters and the measurement channel.