diff --git a/2d_spots_in_fibers.py b/2d_spots_in_fibers.py index 53df4a20310c7de8db1e03ee238ccd16312061f3..41ca9e458357aad65807cfbda249bfbd13ce723c 100644 --- a/2d_spots_in_fibers.py +++ b/2d_spots_in_fibers.py @@ -765,7 +765,7 @@ canvas.close() fiber_label_imp.show() # threshold DAPI channel and convert to binary -dapi_channel = BFopen_image(path_to_image, dapi_channel_number, series_number, z_slice_number=dapi_channel_zslice) +dapi_channel = BFopen_image(path_to_image, dapi_channel_number, series_number) if threshold <= 0: threshold = get_threshold_from_method(dapi_channel, "otsu") dapi_binary = convert_to_binary(dapi_channel, threshold)