diff --git a/README.md b/README.md
index 850f04b4ea311ea8b0604bf833ae2ff87df96c0a..9d7e481769f3dae9262caa93e3c40a02553810b5 100644
--- a/README.md
+++ b/README.md
@@ -40,6 +40,11 @@ Original code: <https://github.com/Hyojung-Choo/Myosoft/tree/Myosoft-hub>
 - Includes identification of double and triple positive combinations.
 - The ROI color code is annotated in the results table.
 
+## [`2d_spots_in_fibers.py`](2d_spots_in_fibers.py)
+
+- Identifies spots in fibers in all desired channels Fiber ROI-zip
+- Includes classification into of DAPI positive or DAPI negative spots
+
 ## [`3_manual_rerun.py`](3_manual_rerun.py)
 
 - Requires an already open image with an already populated ROI manager.