diff --git a/2d_spots_in_fibers.py b/2d_spots_in_fibers.py
index 4e0a81636e3d9dec4e242499d9a2948488d06244..d82f49e263d6145448d81e647a827bb8f9166429 100644
--- a/2d_spots_in_fibers.py
+++ b/2d_spots_in_fibers.py
@@ -6,8 +6,8 @@
 #@ File (label="Fiber Segmentation", description="select the Fiber Segmentation RoiSet .zip file", style=file) fiber_segmentation_roiset
 #@ Integer (label="DAPI channel", description="select the DAPI channel", min=1, max=20, stepSize=1, persist=true, style=slider) dapi_channel_number
 #@ Integer (label="DAPI threshold", description="0 = Auto", min=0) threshold
-#@ String (label="processing channels", description="comma separated list of channels, e.g. 2,3,6,7", value="1,2,3,4") processing_channels_string
-#@ String (label="Spot detection quality threshold", description="comma separated list of values, one per channel", value="80,80,80,80" ) quality_thresholds_string
+#@ String (label="processing channels", description="comma separated list of channels, e.g. 2,3,6,7", value="3,4") processing_channels_string
+#@ String (label="Spot detection quality threshold", description="comma separated list of values, one per channel", value="120,120" ) quality_thresholds_string
 #@ Double (label="Minimum spot diameter", description="smaller spots will be discarded", value=0.3, min=1, max=10, stepSize=0.1) spot_diameter
 
 # trackmate imports