From 1bf4e8d9c532eea3e6429ed5a691f6c588b21aa8 Mon Sep 17 00:00:00 2001
From: Niko Ehrenfeuchter <nikolaus.ehrenfeuchter@unibas.ch>
Date: Mon, 3 Sep 2018 17:52:00 +0200
Subject: [PATCH] Prevent saturation from binning when converting to 8 bit
---
.../scripts/Plugins/NoiSee/NoiSee_Bead_Analysis.ijm | 11 ++---------
1 file changed, 2 insertions(+), 9 deletions(-)
diff --git a/src/main/resources/scripts/Plugins/NoiSee/NoiSee_Bead_Analysis.ijm b/src/main/resources/scripts/Plugins/NoiSee/NoiSee_Bead_Analysis.ijm
index 5a8cf10..14c23cb 100644
--- a/src/main/resources/scripts/Plugins/NoiSee/NoiSee_Bead_Analysis.ijm
+++ b/src/main/resources/scripts/Plugins/NoiSee/NoiSee_Bead_Analysis.ijm
@@ -666,15 +666,8 @@ function process_beads() {
if (bitDepth() == 16) {
print("16 bit image type detected, converting to 8 bit...");
- Stack.getStatistics(_, _, stack_min, stack_max, _);
- new_max = stack_max * 1.1;
- // NOTE: having a new value larger than the max value of the current bit
- // depth is unproblematic since ImageJ apparently just uses the maximum
- // possible value then:
- print("setting display range min/max to 0/" + new_max + " (was: " +
- stack_min + "/" + stack_max + ")");
- setMinAndMax(0, new_max);
- run("8-bit");
+ valueRange = detectValueRange();
+ mapTo8bitPreservingSaturation(valueRange[0]);
}
// remove the scaling so all units (measurements, coordinates, ...) are pixel-based:
--
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