diff --git a/workflow/rules/paired_end.snakefile.smk b/workflow/rules/paired_end.snakefile.smk index c444f461b08af8aa28b5e74ef2bbe315eaefea27..096f8958d1cb7d365af242ebbc30ede7c1701ab0 100644 --- a/workflow/rules/paired_end.snakefile.smk +++ b/workflow/rules/paired_end.snakefile.smk @@ -173,7 +173,6 @@ rule pe_remove_polya_cutadapt: -n 2 \ -e 0.1 \ -q 6 \ - -m 10 \ -a {params.polya_3_mate1} \ -A {params.polya_3_mate2} \ -o {output.reads1} \ diff --git a/workflow/rules/single_end.snakefile.smk b/workflow/rules/single_end.snakefile.smk index d2df8889152c15402d21b8d0dd9f67ce8bd39cda..5f9372be2236c5a81bf0b0f169d42f0d14e87c34 100644 --- a/workflow/rules/single_end.snakefile.smk +++ b/workflow/rules/single_end.snakefile.smk @@ -15,10 +15,6 @@ rule fastqc: "{sample}", "mate1_fastqc")) - params: - seqmode = lambda wildcards: - samples_table.loc[wildcards.sample, "seqmode"] - singularity: "docker://zavolab/fastqc:0.11.9-slim" @@ -232,7 +228,7 @@ rule map_genome_star: --outFilterMatchNminOverLread 0.3 \ --outFilterType BySJout \ --outReadsUnmapped None \ - --outSAMattrRGline ID:rcrunch SM:{params.sample_id} \ + --outSAMattrRGline ID:rnaseq_pipeline SM:{params.sample_id} \ --alignEndsType {params.soft_clip} > {output.bam};) \ 2> {log.stderr}"