Salmon: indexing genome and transcriptome
With salmon 1.0.0+ we should prepare and index genome+transcriptome in the following manner:
https://combine-lab.github.io/alevin-tutorial/2019/selective-alignment/
Please take a look here: https://www.biorxiv.org/content/10.1101/138800v2
Authors also emphasize this on salmon gitter channel.
Edit:
That is also what they recommend in the docs (section 3.2):
https://readthedocs.org/projects/salmon/downloads/pdf/latest/
Edited by BIOPZ-Bak Maciej