cDNA Generator module

Description of the module: The function of this module is to generate cdDNA based on mRNA transcript seuqences and the coresponding priming probabilities.

Input files

transcript_copies (csv-formatted) containing:

• ID of transcript
• ID of parent transcript
• transcript copy number

transcript_sequences (fasta-formatted) containing:

• ID of transcript
• transcript-sequence

priming_sites (gtf-formatted) containing:

• ID of transcript
• Position of priming site
• Binding likelihood of priming site

Output files

cDNA_sequences (fasta-formatted) containing:

• cDNA sequence ID
• cDNA-sequence

cDNA_counts (csv-formatted) containing:

• cDNA sequence ID
• cDNA-counts