Project-Design.md

@@ -26,4 +26,8 @@ Simulating cDNA synthesis This is done by reverse transcribing starting from the
 - CGGGGCG… 40
 
 4. Iterate 1-3 and extend lists
-5. Write unique_transcripts output file and copy_number_transcripts output file
\ No newline at end of file
+5. Write unique_transcripts output file and copy_number_transcripts output file
+
+**Open questions:**
+
+* What if the <span dir="">RT-polymerase</span> is breaking off before reaching the 5'-end of the transcript? With the current design we only consider potential start sites (priming sites).
\ No newline at end of file