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Update Project Design
authored
Oct 19, 2022
by
Bastian Wagner
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@@ -6,14 +6,14 @@ Simulating cDNA synthesis This is done by reverse transcribing starting from the
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@@ -6,14 +6,14 @@ Simulating cDNA synthesis This is done by reverse transcribing starting from the
**Design**
**Design**
1.
Extract transcritpt_sequence, transcritpt_copy_number, priming_sites and priming_probabilities from input files.
1.
Extract transcritpt_sequence
s
, transcritpt_copy_number, priming_sites and priming_probabilities from input files.
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transcritpt_sequence = GATGCGG…
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transcritpt_sequence
s
= GATGCGG…
, AAGCGCGG…, CTCTTGCGG…
\[
...
\]
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transcritpt_copy_number = 100
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transcritpt_copy_number = 100
, 40, 30
\[
...
\]
-
priming_sites = 220, 260, 390
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priming_sites = 220, 260, 390
\[
...
\]
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priming_probabilities = 0.33, 0.27, 0.40
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priming_probabilities = 0.33, 0.27, 0.40
\[
...
\]
2.
Generate a list of unique_transcripts based on transcritpt_sequence + priming_sites and add the list to the fasta output file.
2.
Generate a list of unique_transcripts based on transcritpt_sequence
s
+ priming_sites and add the list to the fasta output file.
-
TTTACGGT…
-
TTTACGGT…
-
CCATACGG…
-
CCATACGG…
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