Simulating cDNA synthesis This is done by reverse transcribing starting from the primer sequence. For each transcript we have the sequence and the copy number. So we for each copy of the transcript we have to sample a priming site in proportion to its probability, calculated at the previous step. Then the cDNAs will be all the sequences generated from the initial pool of transcripts by copying the initial transcript sequence up to the chosen priming site.
Simulating cDNA synthesis This is done by reverse transcribing starting from the primer sequence. For each transcript we have the sequence and the copy number. So we for each copy of the transcript we have to sample a priming site in proportion to its probability, calculated at the previous step. Then the cDNAs will be all the sequences generated from the initial pool of transcripts by copying the initial transcript sequence up to the chosen priming site.
