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Predicting translation initiation efficiency
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zavolan_group
data_analysis
Predicting translation initiation efficiency
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5b073670
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5b073670
authored
7 months ago
by
Niels Schlusser
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Typesetting corrections README 2
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@@ -13,12 +13,14 @@ There are deep learning scripts for essentially three different usecases:
-
MPRA from Sample et.al. (2019)
-
endogenous (riboseq/RNAseq) data based on Alexaki et.al. (2020)
-
clinvar variations based on Landrum et. al. (2020)
are provided in the directory HEK293_training_data/
## Scripts
1.
turn the output of RNAseq and ribosome profiling data into translation efficiency estimates
2.
append non-sequential features to a given data set
3.
construct a data set based on a vcf file
can be found in the directory training_data_preprocessing/.
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@@ -30,12 +32,14 @@ The preprocessing procedure for MPRA data calculates and appends the non-sequent
-
number_inframe_uAUGs
-
normalized_5p_folding_energy
-
GC_content
to the input file using.
### Endogenous data
The preprocessing procedure for endogenous data takes mapping files from:
-
riboseq data analysis
-
RNAseq data analysis
as
*input*
and turns it into a file with:
-
translation efficiencies
-
5'UTR sequences
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@@ -49,6 +53,7 @@ As an input from the riboseq side, you need:
-
bam and bai file of the mapping done in riboseq
-
an alignment json file that contains the p-site offsets for different RPF lengths
-
a tsv file that links gene id and transcript id
From the RNA seq side, you need
-
transcripts_numreads.tsv (output from kallisto)
-
a file with the TIN scores (potentially per replicate)
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@@ -86,6 +91,7 @@ There are a few parameters to specify in the middle of the script:
-
the path to the directory where to save the scalers
-
the path for saving the trained model
-
the path for the pretrained model (for transfer learning, only)
All these scripts can be run in
1.
normal mode (training and testing)
2.
with the suffix 'predict' after '''python3
<script
name
>
''' for prediction and scatterplot creation, only
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