-
In labkey_to_snakemake.py fixed the parameters so that there is 3p as well 5p polya feature for every mate, which can be matched to the -a -g -A and -G options of cutadapt depending on which is the sense or antisense mate the appropriate variable is populated and the rest of variables are filled with 'XXXXXXXXXXXX' which leads to no trimming by cutadapt. The poly-A trimming rules are fixed to contain all -a -g -A -G options.
In labkey_to_snakemake.py fixed the parameters so that there is 3p as well 5p polya feature for every mate, which can be matched to the -a -g -A and -G options of cutadapt depending on which is the sense or antisense mate the appropriate variable is populated and the rest of variables are filled with 'XXXXXXXXXXXX' which leads to no trimming by cutadapt. The poly-A trimming rules are fixed to contain all -a -g -A -G options.
samples.tsv 1.20 KiB
sample seqmode fq1 index_size kmer fq1_3p fq1_5p organism gtf gtf_filtered genome tr_fasta_filtered sd mean multimappers soft_clip pass_mode libtype fq1_polya_3p fq1_polya_5p kallisto_directionality fq2 fq2_3p fq2_5p fq2_polya_3p fq2_polya_5p
synthetic_10_reads_paired_synthetic_10_reads_paired paired_end ../input_files/project1/synthetic.mate_1.fastq.gz 75 31 AGATCGGAAGAGCACA XXXXXXXXXXXXX homo_sapiens ../input_files/homo_sapiens/annotation.gtf ../input_files/homo_sapiens/annotation.gtf ../input_files/homo_sapiens/genome.fa ../input_files/homo_sapiens/transcriptome.fa 100 250 10 EndToEnd None A AAAAAAAAAAAAAAAAA XXXXXXXXXXXXXXXXX --fr ../input_files/project1/synthetic.mate_2.fastq.gz AGATCGGAAGAGCGT XXXXXXXXXXXXX XXXXXXXXXXXXXXXXX TTTTTTTTTTTTTTTTT
synthetic_10_reads_mate_1_synthetic_10_reads_mate_1 single_end ../input_files/project2/synthetic.mate_1.fastq.gz 75 31 AGATCGGAAGAGCACA XXXXXXXXXXXXX homo_sapiens ../input_files/homo_sapiens/annotation.gtf ../input_files/homo_sapiens/annotation.gtf ../input_files/homo_sapiens/genome.fa ../input_files/homo_sapiens/transcriptome.fa 100 250 10 EndToEnd None A AAAAAAAAAAAAAAAAA XXXXXXXXXXXXXXXXX --fr XXXXXXXXXXXXX XXXXXXXXXXXXX XXXXXXXXXXXXX XXXXXXXXXXXXX XXXXXXXXXXXXX