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Transcript sampler
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zavolan_group
tools
Transcript sampler
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5f0e6b1b
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5f0e6b1b
authored
2 years ago
by
Hugo Gillet
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# Transcript Sampler
This workflow takes as input:
-
genome annotation gtf file
-
expression levels of each gene
-
csv file with transcript IDs and expression levels
This workflow sample representative transcripts per gene, in proportion to their relative abundance levels. Sampling is done by poisson sampling.
**This workflow takes as input:**
-
Path to genome annotation file in gtf format
-
Integer of number of transcripts to sample
-
Path to csv or tsv file with transcript IDs and expression levels
-
Path to output sample gtf file
-
Path to output sample transcript IDs and counts
The output is a trancript sample gtf file and csv file containing transcript IDs and counts.
**The outputs are :**
-
trancript sample gtf file
-
csv file containing sample transcript IDs and counts.
The workflow can be run via the command line as
**
The workflow can be run via the command line as
**
python scripts/new-exe.py --annotation {gtf input file} --output_csv {output csv file} --transcript_number {number of transcripts} --output_gtf {output gtf file} --input_csv {input csv file}
`python scripts/new-exe.py --annotation {gtf input file} --output_csv {output csv file} --transcript_number {number of transcripts} --output_gtf {output gtf file} --input_csv {input csv file}`
Exemple :
`python scripts\new_exe.py --annotation "input_files\test.gtf" --output_csv "output_files\output_csv.txt" --transcript_number 50 --output_gtf "output_files\output_gtf.gtf" --input_csv "input_files/expression.csv"`
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