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zavolan_group
pipelines
riboseq_pipeline
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2f8d6036
Commit
2f8d6036
authored
6 years ago
by
BIOPZ-Gypas Foivos
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# Riboseq pipeline
## Requirements
*
wget
*
git
*
singularity
## Features
Pipeline for Ribo-Seq data. It consists of two snakemake workflows:
*
prepare_annotation: Prepares the annotation files
*
process_data: Processes the Ribo-Seq data
## Installation
The recommended way is to create a virtual environment via conda and install the snakemake dependenies.
**In order to run the workflows you need to run it in a system where singularity is available.**
### Step 1: Download miniconda 3 installation file (if not already installed)
for Linux:
```
wget https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
```
### Step 2: Install miniconda 3
Make sure that you run the 'bash' shell and execute:
for Linux:
```
bash Miniconda3-latest-Linux-x86_64.sh
```
### Step 3: Create a new conda environment
Create a new conda environment
```
conda create --name riboseq_pipeline --channel bioconda --channel conda-forge snakemake=4.8.1
```
Activate the virtual environment
```
conda activate riboseq_pipeline
```
You can deactivate later the virtual environment as
```
conda deactivate
```
Check if snakemake was installed properly
```
snakemake --help
```
### Step 4: Clone the repository
```
git clone ssh://git@git.scicore.unibas.ch:2222/AnnotationPipelines/riboseq_pipeline.git
```
## Configure pipeline
ToDo
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