adding cDNA generation script
solving #5
Merge request reports
Activity
requested review from @zavolan
- I read the transcript ID and transcript sequence from fasta-file
- search in gtf-file for transcript ID to find all priming sites for that transcript
- search in csv-file for transcript ID to find the count of transcript
- create cDNAs: loop over the counts of a given transcript, select a priming site in proportion to their relative probabilities and generate the DNA that starts at the transcript start and ends at the chosen priming site 5a. write output fasta-file: cDNA ID and cDNA sequence 5b. write output csv-file: "cDNA ID, Gene ID, Count of cDNA"
Edited by MihaelaZavolan
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