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zavolan_group
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mirflowz
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4b3080c5
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4b3080c5
authored
2 years ago
by
Iris Mestres Pascual
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chore: remove unused config file
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b0c39e14
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chore: remove unused config file
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workflow/config.yaml
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---
#### GLOBAL PARAMETERS #####
# Directories
# Usually there is no need to change these
map_input_dir
:
"
path/to/map_input_directory"
# For the mapping worflow
quantify_input_dir
:
"
path/to/quantify_input_directory"
# For the quantify worflow
output_dir
:
"
results"
scripts_dir
:
"
../scripts"
local_log
:
"
logs/local"
cluster_log
:
"
logs/cluster"
# Inputs information
sample
:
[
"
sample_1"
,
"
sample_2"
]
# put all sample names, separated by comma
#######################################################################################################
####
#### PREPARE PARAMETERS
####
#######################################################################################################
# Isomirs annotation file
# Number of base pairs to add/substract from 5' (start) and 3' (end) coordinates.
bp_5p
:
[
0
]
# array of numbers, e.g., [-2,-1,0,+1], to include 2 upstream and 1 downstream nts
bp_3p
:
[
0
]
# array of numbers, e.g., [-2,-1,0,+1], to include 2 upstream and 1 downstream nts
# List of inputs
organism
:
[
"
org/pre"
]
# e.g., ["homo_sapiens/GRCh38.100", "mus_musculus/GRCm37.98"]
# this string specifies a path, and the "/" is important for this
# "pre" specifies the assembly version
#### PARAMETERS SPECIFIC TO INPUTS #####
org/pre
:
# One section for each list item in "organism"; entry should match precisely what
# is in the "organism" section above, one entry per list item above, omitting the ""
# URLs to genome, gene & miRNA annotations
genome_url
:
# FTP/HTTP URL to gzipped genome in FASTA format, Ensembl style
# e.g. "ftp://ftp.ensembl.org/pub/release-106/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna_sm.primary_assembly.fa.gz"
gtf_url
:
# FTP/HTTP URL to gzipped gene annotations in GTF format, Ensembl style
# e.g. "ftp://ftp.ensembl.org/pub/release-106/gtf/homo_sapiens/Homo_sapiens.GRCh38.106.chr.gtf.gz"
mirna_url
:
# FTP/HTTP URL to unzipped microRNA annotations in GFF format, miRBase style
# e.g. "https://www.mirbase.org/ftp/CURRENT/genomes/hsa.gff3"
# Chromosome name mappings between UCSC <-> Ensembl
# Other organisms available at: https://github.com/dpryan79/ChromosomeMappings
map_chr_url
:
# FTP/HTTP URL to mapping table
# e.g. "https://raw.githubusercontent.com/dpryan79/ChromosomeMappings/master/GRCh38_UCSC2ensembl.txt"
# Chromosome name mapping parameters:
column
:
1
# Column number from input file where to change chromosome name
delimiter
:
"
TAB"
# Delimiter of the input file
#######################################################################################################
####
#### MAP PARAMETERS
####
#######################################################################################################
# Resources: genome, transcriptome, genes, miRs
# All of these are produced by the "prepare" workflow
genome
:
"
path/to/genome.processed.fa"
gtf
:
"
path/to/gene_annotations.filtered.gtf"
transcriptome
:
"
path/to/transcriptome_idtrim.fa"
transcriptome_index_segemehl
:
"
path/to/transcriptome_index_segemehl.idx"
genome_index_segemehl
:
"
path/to/genome_index_segemehl.idx"
exons
:
"
path/to/exons.bed"
header_of_collapsed_fasta
:
"
path/to/headerOfCollapsedFasta.sam"
# Tool parameters: quality filter
q_value
:
10
# Q (Phred) score; minimum quality score to keep
p_value
:
50
# minimum % of bases that must have Q quality
# Tool parameters: adapter removal
error_rate
:
0.1
# fraction of allowed errors
minimum_length
:
15
# discard processed reads shorter than the indicated length
overlap
:
3
# minimum overlap length of adapter and read to trim the bases
max_n
:
0
# discard reads containing more than the indicated number of N bases
# Tool parameters: mapping
max_length_reads
:
30
# maximum length of processed reads to map with oligomap
nh
:
100
# discard reads with more mappings than the indicated number
#### PARAMETERS SPECIFIC TO INPUTS ####
sample_1
:
# one section per list item in "sample"; names have to match
adapter
:
"
XXXXXXXXXXXXXXXXXXXX"
# 3' adapter sequence to trim
format
:
"
fa"
# file format; currently supported: "fa"
#######################################################################################################
####
#### QUANTIFY PARAMETERS
####
#######################################################################################################
# Types of miRNAs to quantify
# Remove miRNA types you are not interested in
mir_list
:
[
"
miRNA"
,
"
miRNA_primary_transcript"
,
"
isomirs"
]
# Resources: miR annotations, chromosome name mappings
# All of these are produced by the "prepare" workflow
mirnas_anno
:
"
path/to/mirna_filtered.bed"
isomirs_anno
:
"
path/to/isomirs_annotation.bed"
...
\ No newline at end of file
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