- Mar 25, 2020
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BIOPZ-Bak Maciej authored
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- Mar 21, 2020
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Alex Kanitz authored
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- Mar 20, 2020
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- generate nucleotide distribution for unique reads only - new rule to generate PNG image for MultiQC
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- Mar 19, 2020
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- Mar 12, 2020
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BIOPZ-Bak Maciej authored
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BIOPZ-Bak Maciej authored
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Dominik Burri authored
moved input_files into top-layer test directory for consistency. corrected removal of test files
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Dominik Burri authored
corrected md5sum for config.yaml remove unnecessary file
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Dominik Burri authored
- renaming bedgraph - creating ALFA qc plots removed conda dependence, moved import statement. included ALFA in finish rule, corrected annotation.gtf and config.yaml, created new .svg
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- Mar 06, 2020
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BIOPZ-Gypas Foivos authored
Merged paired end and single end rules for star_rpm and index_genomic_alignment_samtools. Fixed wiring of calculate tin score: bam should be input and not params.
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- Feb 24, 2020
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- compile Salmon gene and transcript count summary tables across all samples in workflow run - add `pandas` to `install/environment.dev.yml` - update rule graph and DAG images
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- Feb 21, 2020
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Alex Kanitz authored
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- Remove files with non-deterministic output from `tests/test_integration_workflow/expected_output.files` - Update MD5 sums in `tests/test_integration_workflow/expected_output.md5` - Update new workflow DAG and rule graph images
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- fixes some functions in `labkey_to_snakemake.py` - add optional argument for trimming polyA tails; they are trimmed as follows: - if mate is sense, oligo-A is added to sample table for `cutadapt` rule to trim - if mate is antisense, oligo-T is added to sample table for `cutadapt` rule to trim - if option is set to `--trim_polya`, oligo-X stretch is added to sample table and `cutadapt` will not trim
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- Feb 17, 2020
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- add rule for input preparation (GTF to BED12) - add rule for TIN score calculation - update rule graph and DAG image - update Slurm cluster config
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- Feb 15, 2020
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- add script that prepares Snakemake input files 'samples.tsv' and 'config.yaml' from LabKey table - script either connects to API directly (with '--remote' and related options) or processes a tab-separated LabKey dump file - add tests for both use cases - common input files for tests now in 'tests/input_files' - update all other tests to account for new file locations - update documentation
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- Feb 14, 2020
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- separate organism genome architecture (different input folder) - change MD5 checksums to match the new output
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- add script `tests/test_rule_graph/test.sh` to generate a rule graph in `images/rule_graph.svg` - display rule graph created in `README.md` instead of specific workflow DAG - add test script to GitLab CI config - renamed test to create workflow DAG from `test_create_dag_chart` to `test_create_dag_image` (also output file is renamed from `images/workflow_dag.svg` to `images/dag_test_workflow.svg`
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- Feb 09, 2020
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Alex Kanitz authored
- replaces existing larger libraries and annotations in test cases `test_create_dag_chart` and `test_integration_workflow` - adds the following new test files: - `chr1-10000-20000.fa`: artificial chromosome of length 10'000 (based on human chromosome 1) - `chr1-10000-20000.gtf`: matching gene annotation file with two gene and three multi-exon transcripts entries - `chr1-10000-20000.transcripts.fa`: sequences of the transcripts listed in the gene annotation file - `synthetic.mate_?.fastq.gz`: 10 read pairs randomly sampled from the genic regions of the artificial chromosome - `synthetic.*.bed`: BED files with expected alignments for each read; names of overlapping genes are specified in a 7th column - updates file paths in the relevant sample tables - extends and updates checksum checking of result files in CI/CD pipeline
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- Feb 08, 2020
- Feb 04, 2020
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Alex Kanitz authored
- set up integration test for Snakefile in dedicated folder; current test case was left untouched for the time being, despite requiring large input files - set up DAG chart creation test in dedicated folder; script creates an SVG representation of the workflow DAG at `images/workflow_dag.svg` - both tests have been added to the GitLab CI/CD configuration; the latter test ensures that always the latest version of the - all tests are now located inside subdirectories of `tests/`; test scripts and configuration files for test runs etc. have been moved to the appropriate test directories - for the time being, required input files for each test are placed within the individual test directories; a layout for common test files should be introduced later and paths and bind paths in tests adapted - make script `scripts/labkey_api.py` executable
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