- May 07, 2021
-
-
BIOPZ-Gypas Foivos authored
Use biocontainers images for star, gffread, salmon, kallisto, cutadapt, samtools, fastqc, alfa, bedtools, bedgraphtobigwig. Change container from bash to ubuntu. Fixes #149
-
- Feb 26, 2021
-
-
- Feb 11, 2021
-
-
- Jun 23, 2020
-
-
The rules rely on https://github.com/zavolanlab/merge_kallisto Update info in pipeline_documentation.md
-
- Apr 27, 2020
-
-
* Sequencing mode-related changes: * allowed sequencing modes in Snakemake input table changed from `paired_end` and `single_end` to `pe` and `se`, respectively * remove sequencing mode from output paths for each rule * corresponding wild cards removed entirely from all rules that do not depend on sequencing mode (currently all rules that are defined in the main `Snakefile` in the project root directory) * where absolutely necessary, sequencing mode is added as part of output file or directory instead * remove dependency of sequencing mode for rule for `FastQC`; now runs separately for each strand * Changes related to MultiQC and output file/directory structure * moving and renaming outputs for MultiQC is no longer required * code to create MultiQC custom config externalized into script `scripts/rhea_multiqc_config.py` * add MultiQC output files with deterministic output to md5 sum checks performed during execution of `tests/test_integration_workflow/test.{local,slurm}.sh` * output filenames for each rule now follow this general structure: `samples/{sample_name}/{rule}/{output_file}` * change log directory structure matches results directory structure * Miscellaneous changes * consistent, PEP8-compliant formatting in most parts, including Snakemake files, where allowed * remove rule `extract_decoys_salmon`; equivalent file `chrName.txt` produced by `star_index` is used instead * add rule `start` which copies sample data to the results directory and enforces uniform naming * refactoring of ALFA rules and modification of the CI/CD test to ensure compatibility
-
-
- Mar 25, 2020
-
-
- Mar 20, 2020
-
-
- generate nucleotide distribution for unique reads only - new rule to generate PNG image for MultiQC
-
In labkey_to_snakemake.py fixed the parameters so that there is 3p as well 5p polya feature for every mate, which can be matched to the -a -g -A and -G options of cutadapt depending on which is the sense or antisense mate the appropriate variable is populated and the rest of variables are filled with 'XXXXXXXXXXXX' which leads to no trimming by cutadapt. The poly-A trimming rules are fixed to contain all -a -g -A -G options.
-
- Mar 19, 2020
-
-
- Mar 17, 2020
-
-
- Mar 12, 2020
-
-
Dominik Burri authored
corrected md5sum for config.yaml remove unnecessary file
-
- Mar 06, 2020
-
-
BIOPZ-Gypas Foivos authored
-
-
-
- Feb 21, 2020
-
-
Alex Kanitz authored
-
- Remove files with non-deterministic output from `tests/test_integration_workflow/expected_output.files` - Update MD5 sums in `tests/test_integration_workflow/expected_output.md5` - Update new workflow DAG and rule graph images
-
- Feb 15, 2020
-
-
- add script that prepares Snakemake input files 'samples.tsv' and 'config.yaml' from LabKey table - script either connects to API directly (with '--remote' and related options) or processes a tab-separated LabKey dump file - add tests for both use cases - common input files for tests now in 'tests/input_files' - update all other tests to account for new file locations - update documentation
-
- Feb 09, 2020
-
-
Alex Kanitz authored
- replaces existing larger libraries and annotations in test cases `test_create_dag_chart` and `test_integration_workflow` - adds the following new test files: - `chr1-10000-20000.fa`: artificial chromosome of length 10'000 (based on human chromosome 1) - `chr1-10000-20000.gtf`: matching gene annotation file with two gene and three multi-exon transcripts entries - `chr1-10000-20000.transcripts.fa`: sequences of the transcripts listed in the gene annotation file - `synthetic.mate_?.fastq.gz`: 10 read pairs randomly sampled from the genic regions of the artificial chromosome - `synthetic.*.bed`: BED files with expected alignments for each read; names of overlapping genes are specified in a 7th column - updates file paths in the relevant sample tables - extends and updates checksum checking of result files in CI/CD pipeline
-
- Feb 08, 2020
- Feb 07, 2020
-
-
Alex Kanitz authored
- remove log files and add '.snakemake' directories to '.gitignore' - update wrong link in 'README.md' - delete superfluous script documentation 'scripts/labkey_api.md' - add Snakemake-specific file extension '.smk' to subworkflows - remove non-deterministic workflow output from md5 sums
-
- Feb 04, 2020
-
-
Alex Kanitz authored
- set up integration test for Snakefile in dedicated folder; current test case was left untouched for the time being, despite requiring large input files - set up DAG chart creation test in dedicated folder; script creates an SVG representation of the workflow DAG at `images/workflow_dag.svg` - both tests have been added to the GitLab CI/CD configuration; the latter test ensures that always the latest version of the - all tests are now located inside subdirectories of `tests/`; test scripts and configuration files for test runs etc. have been moved to the appropriate test directories - for the time being, required input files for each test are placed within the individual test directories; a layout for common test files should be introduced later and paths and bind paths in tests adapted - make script `scripts/labkey_api.py` executable
-