refactor: rename LabKey/input table column

See merge request !70

Add Snakemake report

See merge request !64

docs: rename project/workflow

See merge request !69

fix(prepare_inputs): support relative paths

See merge request !68

Salmon argument fix

See merge request !67

BIOPZ-Katsantoni Maria authored 4 years ago and BIOPZ-Gypas Foivos committed 4 years ago

fix: added arguments for fragment size mean and sd required for the salmon quantification approach in single-end mode

Gtf2bed update

See merge request !65

Extend workflow description

See merge request zavolan_group/pipelines/rhea!63

- add linked ToC
- add table of third party software
- add rule graph
- clean up sample table description
- formalize structure for each rule description
- cross-reference rules via inputs/outputs
- clean list of references

Major refactoring before release

Closes #117, #112, #111, #108, #107, #101, and #74

See merge request zavolan_group/pipelines/rhea!62

- clean up command line interface
  - improve descriptions
  - add consistent structure
  - remove or merge superfluous CLI arguments
  - set defaults
  - update test calls
  - update docs
  - when importing data from LabKey, table is saved to 'samples.tsv.labkey' in same directory as Snakemake sample table
- allow user to specify environment variables and relative paths in input table and on CLI
  - relative paths in the input table are interpreted with respect to the directory containing the input table
  - relative paths will are interpreted with respect to the current working directory; this is to achieve portability with respect to tests but is discouraged in production because its behavior is not very predictable from the user's perspective; consequently a warning is thrown
- set STAR index size to read length - 1
- remove `gtf_filtered` and `tr_fasta_filtered` and update Snakefiles and test sample tables accordingly
- rename some MultiQC report-related parameters and update Snakefiles and test config files accordingly
- add logging
- add docstrings to module and all functions
- add typing definitions to all functions
- restructure and comment code to improve readability
- linters `flake8` and `mypy` pass

BIOPZ-Katsantoni Maria authored 4 years ago and Alex Kanitz committed 4 years ago

* Sequencing mode-related changes:
  * allowed sequencing modes in Snakemake input table changed from `paired_end` and `single_end` to `pe` and `se`, respectively
  * remove sequencing mode from output paths for each rule
  * corresponding wild cards removed entirely from all rules that do not depend on sequencing mode (currently all rules that are defined in the main `Snakefile` in the project root directory)
  * where absolutely necessary, sequencing mode is added as part of output file or directory instead
  * remove dependency of sequencing mode for rule for `FastQC`; now runs separately for each strand
* Changes related to MultiQC and output file/directory structure
  * moving and renaming outputs for MultiQC is no longer required
  * code to create MultiQC custom config externalized into script `scripts/rhea_multiqc_config.py`
  * add MultiQC output files with deterministic output to md5 sum checks performed during execution of `tests/test_integration_workflow/test.{local,slurm}.sh`
  * output filenames for each rule now follow this general structure: `samples/{sample_name}/{rule}/{output_file}`
  * change log directory structure matches results directory structure
* Miscellaneous changes
  * consistent, PEP8-compliant formatting in most parts, including Snakemake files, where allowed
  * remove rule `extract_decoys_salmon`; equivalent file `chrName.txt` produced by `star_index` is used instead
  * add rule `start` which copies sample data to the results directory and enforces uniform naming
  * refactoring of ALFA rules and modification of the CI/CD test to ensure compatibility

Update salmon transcriptome index generation

See merge request zavolan_group/pipelines/rhea!59

Second fix in results restructuring

See merge request zavolan_group/pipelines/rhea!57

fix absolute / relative path issue in fastqc results parsing

See merge request zavolan_group/pipelines/rhea!56

update documentation

See merge request zavolan_group/pipelines/rhea!54

Use minified container images

Closes #105

See merge request zavolan_group/pipelines/rhea!55

ALFA merged plots included in the MultiQC report

See merge request zavolan_group/pipelines/rhea!53

ALFA extensions

Closes #100 and #93

See merge request zavolan_group/pipelines/rhea!52

Dominik Burri authored 4 years ago and Alex Kanitz committed 4 years ago

- generate nucleotide distribution for unique reads only
- new rule to generate PNG image for MultiQC

Polya trimming correction

Closes #98

See merge request zavolan_group/pipelines/rhea!50

BIOPZ-Katsantoni Maria authored 4 years ago and Alex Kanitz committed 4 years ago

In labkey_to_snakemake.py fixed the parameters so that there is 3p as well 5p polya
feature for every mate, which can be matched to the -a -g -A and -G options of cutadapt
depending on which is the sense or antisense mate the appropriate variable is populated
and the rest of variables are filled with 'XXXXXXXXXXXX' which leads to no trimming by
cutadapt. The poly-A trimming rules are fixed to contain all -a -g -A -G options.

MultiQC

See merge request zavolan_group/pipelines/rhea!49

Rhea naming

Closes #94

See merge request zavolan_group/pipelines/rhea!51